The determination of phytoplankton using high performance liquid chromatography (HPLC) is detailed in Hooker et al. (2005; doi:10.1364/AO.44.000553) and Ras et al. (2008; doi:10.5194/bg-5-353-2008). Briefly, the pigments of particles retained on GF/F (0.7 µm) filters were extracted at -20°C with 3 mL methanol (100%). The filter samples were then disrupted using a sonicator and clarified one hour later by vacuum filtration through GF/F filters. The extracts were rapidly analyzed within 24h by HPLC with a complete Agilent Technologies system (comprising LC Chemstation software, a degasser, a binary pump, a refrigerated autosampler, a column thermostat and a diode array detector).

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