The data contains water chemistry and spectral catchment NDVI for 14 streams in Zackenberg Valley in Northeast Greenland, sampled summer 2021 from 10th July to 15th September. We collected water samples for measuring water chemistry, and we determined landscape parameters using GIS based tools. The data was collected at three sampling periods in summer 2021 in the Zackenberg Research Station (74°28'N, 20°34'W). The area has a polar tundra climate with mean annual air temperature of -9.1 °C. Water chemistry (i.e. dissolved and particulate nitrogen, phosphorus, carbon; dissolved iron and silicate) and catchment characteristics (i.e. catchment area, altitude, slope, aspect, NDVI, snow cover) was measured for each of the 14 stream sites. Water chemistry samples were collected and analyzed using standard methods, and landscape characteristics were determined using GIS resources. The data was collected in order to study relationships between landscape characteristics and stream water chemistry. The water samples were collected by a team of two people, and the detailed methods are given below.