The RotaFam study is a prospective household study on occurrence and within household spread of viral acute gastroenteritis. During two seasons, 604 households including 2298 participants were enrolled and monitored for ten consecutive weeks. 697 AGE episodes in 358 households (59%) were detected with stool samples for virological evaluation obtained from 610 episodes (87%). An additional control stool sample from a random household member was provided for 570 AGE episodes. In addition, a prevalence survey was conducted in 2016 on 1091 subjects, that yielded a virus in 167 stool samples. The dataset contains detailed information on household characteristics, gastro-intestinal symptoms for each individual per day, clinical and contact data for each acute gastroenteritis episode, results of virological testing by qualitative PCR for norovirus, rotavirus, astrovirus and adenovirus 40/41.