The dataset contains records of the Roe Deer (Capreolus capreolus), a major wild prey of the Iberian Wolf, collected in the lower valley of River Sabor (NE Portugal), in the scope of the long-term Ecological Monitoring Program (PME) of the Baixo Sabor Hydroelectric Dams (AHBS), promoted by EDP – Energias de Portugal, S.A. This dataset includes results of surveys made in and around the area affected by the dam, from June 2010 to April 2014. Sampling was performed in transects distributed along the lower Sabor river valley (and some tributaries), and in rivers Maçãs and Angueira, from the river mouth up to 42 km upstream. The presence of the target species was assessed through faecal pellet surveys, due to difficulties in directly observing the species.