-
Global patterns of 16S rRNA diversity at a depth of millions of sequences per...
The ongoing revolution in high-throughput sequencing continues to democratize the ability of small groups of investigators to map the microbial component of the biosphere. In... -
Knight skin biogeography comparison
Knight_skin_biogeography - Comparison of skin samples from a rat (mammal), cyclostomid (fish), iguana (reptile), bull frog (amphibian) and a bird (pigeon) to determine if the... -
DNA extraction comparison testing 2019-2020
Our DNA extraction protocol is years old, and we want to compare new extraction methods to address outstanding issues. For more details, check out the motivation and... -
Knight_extraction_test
One plate of samples was extracted using several different extraction tools (new, faster) for comparison with the existing pipeline -
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,... -
Plasma derived microbiome of melanoma patients and healthy individuals
The objective of this project was to assess if cell-free microbial DNA could be accurately detected and characterized from plasma of melanoma patients and healthy individuals by... -
Baseline and after stressor salivary corticosterone levels in the saliva and ...
Glucocorticoids have been widely used as a physiological marker of stress, and elevated baseline glucocorticoids levels in vertebrates have been associated with environmental...