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Patterns of microbiome transition along salinity gradients, and importance fo...
We are interested in whether the host fish microbiome is important for host adaptation to alternate osmotic niches. Toward this goal, we seek to describe the patterns of... -
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,... -
The Sponge Microbiome Project
Marine sponges (phylum Porifera) are a diverse, phylogenetically deep-branching clade known for forming intimate partnerships with complex communities of microorganisms. To... -
Habitat conditions reflect compositional differences in microbiome among Cari...
Sea urchins are considered one of the most common benthic grazers in tropical marine ecosystems and can exert a strong in??uence in the community structure. The sea urchins... -
Olympia Oyster Microbiome
Olympia Oyster (Ostrea lurida) populations rapidly declined in Washington over a century ago. Currently, there are efforts by the state of Washington DNR to monitor Ostrea... -
mini PCR: effects of PCR rxn volume and gDNA input validation
part of mackerel paper -
Characterization of the intestinal microbiota of the sea cucumber Holothuria ...
High-throughput 16S rRNA gene sequencing has been used to identify the intestinal microbiota of many animal species, but that of marine invertebrate organisms remains largely... -
2018 Australia fish microbiome study
Atl salmon study ABSTRACT Successful rearing of fish in hatcheries is critical for conservation, recreational fishing, and commercial fishing through wild stock enhancements,... -
Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribe...
combined samples sequenced with primers modified for forward barcoding on the 515f site, extended to include V5 and improved ITS -
DNA extraction comparison testing 2019-2020
Our DNA extraction protocol is years old, and we want to compare new extraction methods to address outstanding issues. For more details, check out the motivation and... -
A simple microbiome in the European common cuttlefish, Sepia officinalis
The European common cuttlefish, Sepia officinalis, is used extensively in biological and biomedical research yet its microbiome remains poorly characterized. We analyzed the... -
3D multi-omic profiling of an assemblage of Hawaiian corals
Samples were taken from species Porities lobata, Montipora capitata, Montipora flabellata and subjected to 16S sequencing for bacterial profiling and LC-MS/MS for metabolomic... -
Global patterns of 16S rRNA diversity at a depth of millions of sequences per...
The ongoing revolution in high-throughput sequencing continues to democratize the ability of small groups of investigators to map the microbial component of the biosphere. In... -
Knight skin biogeography comparison
Knight_skin_biogeography - Comparison of skin samples from a rat (mammal), cyclostomid (fish), iguana (reptile), bull frog (amphibian) and a bird (pigeon) to determine if the... -
Genomic basis of differential aerosolization of bacteria and viruses in sea s...
Airborne bacteria and viruses have direct consequences for atmospheric processes and human health, but little is known about factors controlling their transfer from the ocean to... -
Earth Microbiome Project Multi-omics (EMP500)
The Earth Microbiome Project Multi-omics component (EMP500) involves amplicon and shotgun metagenomic sequencing and metabolomic profiling of over five hundred microbial... -
Microbial effects of livestock manure fertilization on freshwater aquaculture...
The majority of seafood is produced from farming, with most finfish coming from freshwater ponds. Across continents, aquaculture is growing fastest in Africa at 11.7 % annual... -
Evaluation of the safety and efficacy of fecal microbiota transplantations in...
Gastrointestinal disease is a leading cause of morbidity in bottlenose dolphins (Tursiops truncatus) under managed care. Fecal microbiota transplantation (FMT) holds promise as...