27 datasets found

The ongoing revolution in high-throughput sequencing continues to democratize the ability of small groups of investigators to map the microbial component of the biosphere. In...

metagenome sequencing

High-quality (HQ) reference genomes are essential for understanding the phylogeny and function of uncultured microbes in complex microbial ecosystems. However, existing...

Exploring the long-term colonisation and persistence of probiotic-prophylaxis species on the gut microbiome of preterm infants: a pilot study

Microbiome data can be used to identify the differences in the structure of the GM based on the differences in the abundance of GM microbes, and this analysis can be used to...

Metagenome profiling at one-health interface of Kathmandu to investigate antimicrobial resistance, virulence factor genes and their possible horizontal gene transfer patterns

combined samples sequenced with primers modified for forward barcoding on the 515f site, extended to include V5 and improved ITS

A cultured isolate collection from a broad human population

Assessing differences due to high fidelity DNA polymerase to downstream 16S rRNA gene survey results. Variations in number of OTUs and chimera prevalence were found to be high...

Our DNA extraction protocol is years old, and we want to compare new extraction methods to address outstanding issues. For more details, check out the motivation and...

What is already known on this topic</p><p>Two-thirds of Small Bowel Transplantation recipients develop bacteremia post-transplantation. One-third die of sepsis,...

Gut fungal and bacterial sampling in allogeneic bone marrow transplant patients, including the ITS sequencing and 16S sequencing data of the gut flora, and whole genome...

Understanding how the human gut microbiota and host are impacted by probiotic bacterial strains requires carefully controlled studies in humans, and in mouse models of the gut...

This project takes advantage of challenge studies with an important human pathogen, Vibrio cholera, the etiological agent of cholera, which causes severe diarrhea in developed...

Creation of a genome sequence database of live microorganisms found in dietary supplements and cultured foods to be used for strain verification and characterization.

LoopSeq assembled 16S amplicons using Illumina PE reads

The purpose of this study was to reveal the relationship between dietary protein patterns and intestinal microbiota. The intestinal microbiota of volunteers was analyzed by...

Human and mouse gut microbiota Raw sequence reads and metagenomic assembly

Assessing differences due to preservation methods and extraction kit used on downstream 16S rRNA gene survey results. Variations in that were detected tended to be smaller than...

Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,...