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Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,... -
dust metagenome Raw sequence reads
Evaluating the impact of atmospheric depositions on dinitrogen fixation in the Eastern Mediterranean Sea- A mesocosm approach -
Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribe...
combined samples sequenced with primers modified for forward barcoding on the 515f site, extended to include V5 and improved ITS -
DNA extraction comparison testing 2019-2020
Our DNA extraction protocol is years old, and we want to compare new extraction methods to address outstanding issues. For more details, check out the motivation and...