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CRISPR-Cas adaptation against a novel conjugative plasmid in Pyrococcus furiosus
The novel conjugative plasmid pT33.3 is taken up by Pyrococcus furiosus and stimulates a CRISPR-Cas immune response. This data set includes PCR amplicons from Pyrococcus... -
Pyrococcus furiosus COM1 Raw sequence reads
Pyrococcus furiosus MNase protected DNA was sequenced to determine patterns of protein binding to the chromosome, particularly near CRISPR arrays. Histones were identified as a... -
The transcriptional regulator TFB-RF1 activates transcription of a putative A...
Transcription factor B recruiting factor 1 (TFB-RF1 PF1088) is a transcription regulator which activates transcription on archaeal promoters containing weak TFB recognition... -
Pyrococcus furiosus COM1 methylation detection - COM1 epigenomics
Pyrococcus furiosus COM1 methylation detection -
Drakenstein12internal
This is a large scale project with the sequencing co-funded by collaborators at the University of Cape Town and WT Core (I2818). This is a project aimed at deepening our... -
In vitro integration of CRISPR spacers - Pyrococcus furiosus
CRISPR-Cas immune systems rely on short DNA sequences to act as immunological memories of past invaders like phages. New DNA sequences, called spacers, can be integrated into a... -
RiboMeth-seq data from Pyrococcus furiosus
Semiconductor sequencing of alkaline degraded total RNA from Pyrococcus furiosus annotated for ”The 23S ribosomal RNA from Pyrococcus furiosus is circularly permuted” published... -
Exploring prokaryotic transcription using Nanopore-based native RNA sequencing
In this study, we explore native RNA sequencing of bacterial (E. coli) and archaeal (H. volcanii, P. furiosus) transcriptomes employing the Oxford Nanopore sequencing technology. -
Pyrococcus furiosus psiRNAs
Small RNA Sequencing from Pyrococcus furiosus Keywords: Small RNA Analysis Overall design: Analysis of Small RNA from one sample of Pyrococcus furiosus -
CopR is a global regulator of copper homeostasis in Pyrococcus furiosus
In several archaea, the Cu-transporting ATPase and the copper chaperone are arranged in a conserved copper resistance gene cluster (cop), which also contains an additional gene,... -
Pyrococcus furiosus strain:JFW02 Targeted Locus (Loci)
Amplicon sequencing of Pyrococcus furiosus CRISPR arrays -
Pyrococcus furiosus Cas mutants, targeted sequencing of CRISPR loci
Targeted sequencing of CRISPR loci in Pyrococcus furiosus revealed patterns of spacer acquisition. These patterns differ among Cas mutants. -
Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping
N4-acetylcytidine (ac4C) is an ancient and highly conserved RNA modification, present on tRNA, rRNA and recently investigated in eukaryotic mRNA. We report ac4C-seq, a chemical... -
Pyrococcus furiosus primed spacer uptake
In CRISPR-Cas, DNA fragments are captured and used as immunological memories. This uptake process can be primed by recognition of an existing target. To study the mechanism in...
