In this study, we investigated the potential of marine sediment and activated carbon to generate and store electricity. Both internal and external energy supply was validated for storage behavior. Four types of anode electrode compositions were investigated. Two were mixture of different volumes of activated carbon (AC) and mixed with Dutch Eastern Scheldt marine sediment (67% AC, 33% AC) and the others were 100% AC or 100% marine sediment based. Each composition was duplicated. Operating these BES’s under MFC mode with solely marine sediment as the anode electron donor resulted in the creation of a bio-battery system. The recharge time of such bio-battery does depend on the fuel content and its usage.